THE PROTECTIVE EFFECTS OF CHRONIC CAFFEINE TREATMENT ON THE COGNITIVE FUNCTION AND SYNAPTIC PLASTICITY IN ACUTE SLEEP DEPRIVATION

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Title: THE PROTECTIVE EFFECTS OF CHRONIC CAFFEINE TREATMENT ON THE COGNITIVE FUNCTION AND SYNAPTIC PLASTICITY IN ACUTE SLEEP DEPRIVATION
Author: Alhaider, Ibrahim
Abstract: Study objectives : Accumulating evidence has shown that caffeine and sleep deprivation have opposing effects on learning and memory ; therefore , this study was undertaken to provide a detailed account of the effect of chronic , low -dose caffeine treatment on the deleterious effects of sleep loss on hippocampus -dependent learning and memory . Experimental design : We investigated the effects of chronic (4 weeks ) caffeine treatment (0 .3 g /l in drinking water ) on memory impairment in acutely (24 hr ) sleep -deprived rats . Sleep deprivation was induced using the modified multiple platform model . The effects of caffeine on sleep deprivation -induced hippocampus -dependent learning and memory deficits were studied using three approaches : learning and memory performance in the radial arm water maze task ; electrophysiological recordings in the Cornu Ammonis (CA1 ) and dentate gyrus (DG ) regions of the hippocampus ; and western blot analysis to measure the levels of memory - and synaptic plasticity -related signaling molecules . Results : Our results showed that chronic caffeine treatment prevented impairment of hippocampus -dependent learning , short -term memory and early phase - long -term potentiation (E -LTP ) of the CA1 and DG areas in the sleep -deprived rats . In correlation , caffeine treatment prevented a sleep deprivation -associated decrease in the basal levels of phosphorylated calcium /calmodulin -dependent protein kinase II (P -CaMKII ) and brain -derived neurotrophic factor (BDNF ) . In addition , caffeine treatment of sleep -deprived rats increased the levels of P -CaMKII during the expression of E -LTP . The results also showed that chronic caffeine treatment prevented the impairment of long -term memory and late phase -LTP (L -LTP ) in the CA1 and DG regions of the sleep -deprived rats . Additionally , caffeine treatment prevented a sleep deprivation -associated decrease in the basal levels of the phosphorylated cAMP response element binding protein (P -CREB ) as well as total CREB . Treating sleep -deprived rats chronically with caffeine enables multiple high frequency stimulation to increase the levels of P -CREB during L -LTP expression . Conclusions : The results suggest that long -term use of a low dose of caffeine protects against the harmful changes in the basal levels of P -CaMKII , P -CREB and BDNF associated with sleep deprivation and as a result contributes to the revival of hippocampus -dependent learning and memory as well as LTP in the CA1 and DG regions .
URI: http : / /hdl .handle .net /10657 /ETD -UH -2010 -05 -31
Date: 2012-09-27

Citation

THE PROTECTIVE EFFECTS OF CHRONIC CAFFEINE TREATMENT ON THE COGNITIVE FUNCTION AND SYNAPTIC PLASTICITY IN ACUTE SLEEP DEPRIVATION. Doctoral dissertation, University of Houston. Available electronically from http : / /hdl .handle .net /10657 /ETD -UH -2010 -05 -31 .

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