Freezing-induced Microstructural Changes Of Collagen Scaffolds

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Title: Freezing-induced Microstructural Changes Of Collagen Scaffolds
Author: Miller, Jeffrey David
Abstract: Understanding the effects of freezing on the functional properties of tissues is critical to cryopreservation of native and artificial tissues , and prognosis after cryotherapies . Although investigations have been performed to correlate macroscopic properties such as mechanical properties with freezing parameters , the effects are typically tissue -type dependent and no physical understanding is available to explain these effects . Since the mechanical and optical properties of a tissue are based on the characteristics of its extracellular matrix (ECM ) , understanding and quantifying these changes could assist in predicting the response a tissue will have to freezing under different circumstances . In this study , the microstructural changes induced to a type I collagen tissue equivalent construct after freezing to different end temperatures were visualized using macroscopic observation , as well as optical microscopy and scanning electron microscopy . The changes were then quantified using image analysis . The study found that freezing results in a thinner and transparent gel at the macroscopic level . Optical microscopy of the frozen region portrayed apparent "wrinkles" in the matrix , and scanning electron microscopy illustrated a sparser , more disorganized scaffold of fibers , though the fibers appeared to be thicker . Image analysis results indicate that freezing causes a significant increase in matrix porosity and fiber diameter .
URI: http : / /hdl .handle .net /10106 /83
Date: 2007-08-23

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Freezing-induced Microstructural Changes Of Collagen Scaffolds. Available electronically from http : / /hdl .handle .net /10106 /83 .

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