Targeted Delivery Of Carbon Nanotubes To Cancer Cells

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Title: Targeted Delivery Of Carbon Nanotubes To Cancer Cells
Author: Chakravarty, Pavitra
Abstract: CD22 is broadly expressed on human B cell lymphomas . Monoclonal anti -CD22 antibodies (MAbs ) alone , or coupled to toxins , have been used to selectively target these tumors both in severe combined immunodeficient (SCID ) mice with xenografted human lymphomas and in patients . Single -walled carbon nanotubes (CNTs ) attached to antibodies or peptides represent another approach to targeting cancer cells . CNTs convert absorbed near -infrared (NIR ) light into heat , which can thermally ablate cells in the vicinity of the CNTs . We have made MAb -CNT constructs where the MAb was either noncovalently or covalently coupled to CNTs , and investigated their ability to bind specifically to cells and to thermally ablate them after exposure to NIR light . The specific binding of these MAb -CNT constructs to antigen -positive and antigen -negative cells was demonstrated in vitro by using CD22+CD25 - Daudi cells , CD22 -CD25+ phytohemagglutinin (PHA ) -activated normal human peripheral blood mononuclear cells (PBMCs ) and CNTs coupled non -covalently or covalently to either anti -CD22 or anti -CD25 . We then demonstrated that the MAb -CNTs could bind to tumor cells expressing the relevant antigen but not to cells lacking the antigen . Furthermore we showed that , following exposure to NIR light , the cells could be thermally ablated . We also determined the stability of the MAb -CNTs in conditions designed to mimic the in vivo environment , i .e . mouse serum at 37oC .We then use the intrinsic Raman signature of CNTs to study the circulation and tissue distribution of intravenously injected MAb -CNTs in a murine xenograft model of lymphoma in vivo over a period of 24 hrs . We demonstrated that the MAb -CNTs have a short half -life in blood and that most of them are cleared by the reticuloendothelial system (RES ) . In the current embodiment , these constructs would therefore be of limited effectiveness in vivo
URI: http : / /hdl .handle .net /10106 /4959
Date: 2010-07-19


Targeted Delivery Of Carbon Nanotubes To Cancer Cells. Available electronically from http : / /hdl .handle .net /10106 /4959 .

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