Electrical Apparoaches For Selective Detection Of Cancer Biomarker Proteins With Aptamers

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Title: Electrical Apparoaches For Selective Detection Of Cancer Biomarker Proteins With Aptamers
Author: Pachampettai Ramachandran, Priyanka
Abstract: Cancer is a disease that is the central focus of many a researcher's attention . As it is a disease that can spread very rapidly and can be fatal in many cases , its early detection and early treatment can dramatically change mortality . Towards the early detection of the disease , antibodies are most commonly used for the detection of cancer biomarkers . The use of antibodies in diagnostics has proven fruitful in the past . But advancements in molecular biology have introduced aptamers for diagnostic and therapeutic purposes . Aptamers are oligonucleotides or peptides that are usually synthesized by a selective process called SELEX . Aptamers are more advantageous as they are stable over wider physical conditions and have higher selectivity than antibodies . The Epidermal Growth Factor Receptor (EGFR ) is a cell surface protein that plays a pivotal role during the tumorigenesis of the cancer cells . It is over -expressed during the onset and the course of cancer . .A biomarker sensor was designed to electrically detect proteins . For initial studies , the capture of R2Bm protein was demonstrated using double -stranded DNA that binds to it . Towards a practical application of the detection approach , EGFR capture and detection was also done . The EGFR was captured by the anti -EGFR RNA aptamer . To electrically detect the presence of the proteins , a Complementary Metal Oxide Semiconductor chip was fabricated with gold nano -electrodes for sensitive electrical detection of two model proteins . The electrical data obtained showed an increase in current by three orders when protein was captured between nano -electrodes in comparison with the control chips . The presence of the protein and the selective agent was confirmed in both cases . The use of CMOS chips in the detection system makes integration with more detection units more feasible . Designing a microarray for the simultaneous detection of many biomarkers is also possible with this detection . Also , to test the prototype device and technology of guided -more resonance , , the EGFR aptamer was immobilized on a titanium dioxide surface . The technology involves the analysis of shift in wavelength after a binding event . Upon analysis it was found that the protein was indeed bound to the aptamer on the titanium dioxide surface .
URI: http : / /hdl .handle .net /10106 /2061
Date: 2010-03-03


Electrical Apparoaches For Selective Detection Of Cancer Biomarker Proteins With Aptamers. Available electronically from http : / /hdl .handle .net /10106 /2061 .

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