Cytotoxicity Evaluation Of Temperature Sensitive Poly(n-isopropylacrylamide) Nanoparticles For Drug Delivery

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Title: Cytotoxicity Evaluation Of Temperature Sensitive Poly(n-isopropylacrylamide) Nanoparticles For Drug Delivery
Author: Wadajkar, Aniket Sharadrao
Abstract: The objective of this research project was to develop temperature sensitive poly (N -isopropylacrylamide ) (PNIPA ) nanoparticles for drug delivery applications . These nanoparticles were formulated by a radical polymerization method , and their toxicity was studied in vitro using MTS assays and various cell types such as fibroblasts , smooth muscle cells , and endothelial cells . Cytotoxicity results did not show a significant difference in cell survival when cells were exposed to different particle sizes (100 , 300 , and 500 nm ) . In addition , dose studies of 100 nm nanoparticles showed that the cells exposed to PNIPA nanoparticles at concentrations less than or equal to 5 mg /ml were biocompatible . Photomicrographs also showed that cells exposed to PNIPA nanoparticles maintained their normal morphology . Additionally , we improved the biocompatibility of PNIPA nanoparticles using various Pluronic surfactants (L64 , P65 , P85 , and F127 ) instead of the common surfactant , sodium dodecyl sulfate (SDS ) . The MTS assay results of free surfactant toxicity studies showed that Pluronic surfactants , especially F127 and P85 , were more biocompatible than SDS over the studied range of concentrations . We also found that PNIPA nanoparticles synthesized using Pluronic were more biocompatible than those of SDS . Finally , drug release studies were done using Doxorubicin as a drug model . PNIPA nanoparticles synthesized using Pluronic surfactants produced higher amount of Doxorubicin release than PNIPA nanoparticles synthesized using SDS at 37oC over the same period of time . In general , Pluronic P85 and F127 used as surfactants to formulate temperature sensitive PNIPA nanoparticles increase the biocompatibility drug release of PNIPA nanoparticles .
URI: http : / /hdl .handle .net /10106 /1134
Date: 2008-09-17

Citation

Cytotoxicity Evaluation Of Temperature Sensitive Poly(n-isopropylacrylamide) Nanoparticles For Drug Delivery. Available electronically from http : / /hdl .handle .net /10106 /1134 .

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