Phylogeography of the cottonmouth, Agkistrodon piscivorus, using AFLP and venom protein profiles.

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Phylogeography of the cottonmouth, Agkistrodon piscivorus, using AFLP and venom protein profiles.

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dc.contributor.advisor Ammerman, Loren K.
dc.contributor.advisor McCoy, J. Kelly
dc.contributor.author Strickland, Jason L.
dc.contributor.other Angelo State University. Department of Biology.
dc.creator Strickland, Jason
dc.date.accessioned 2011-05-25T14:32:04Z
dc.date.available 2011-05-25T14:32:04Z
dc.date.created April 2011
dc.date.issued 2011-05-25T14:32:04Z
dc.identifier.uri http://hdl.handle.net/2346.1/17
dc.description.abstract The objective of this study was to examine population structure in cottonmouths (Agkistrodon piscivorus) using Amplified Fragment Length Polymorphism (AFLP) and compare genetic and venom protein profiles in Texas. AFLP profiles using 622 fragments were generated for 105 individuals to understand the level of variation within Agkistrodon. In Texas, there was a significant lack of gene flow detected and support for the isolation of Concho Valley individuals. Cottonmouths showed the greatest genetic variation when compared to other Agkistrodon species but there was not complete support for two species of cottonmouths as currently proposed. RP-HPLC was used to examine venom protein profiles in 86 Texas cottonmouths. Relative peak heights were analyzed using PCA and the MANOVA demonstrated separation of populations based on profiles (p<0.001). Genetic and venom variation did not follow the same pattern indicating that there may be other selection pressures acting on the venom proteins. en
dc.format.mimetype application/pdf
dc.language.iso en_US en
dc.subject Cottonmouth en
dc.subject Agkistrodon Piscivorus en
dc.subject Venom Protein en
dc.subject Amplified Fragment Length Polymorphism en
dc.subject AFLP en
dc.subject Genetics en
dc.subject.lcsh Agkistrodon piscivorus
dc.subject.lcsh Genetics
dc.subject.lcsh Classification
dc.subject.lcsh Venom
dc.subject.lcsh Research
dc.title Phylogeography of the cottonmouth, Agkistrodon piscivorus, using AFLP and venom protein profiles. en
dc.type Thesis en
dc.type.material text
dc.type.genre Thesis
thesis.degree.name M.S.
thesis.degree.level Masters
thesis.degree.discipline Biology
thesis.degree.grantor Angelo State University
thesis.degree.department Biology
dc.contributor.committeeMember Ross, Linda
dc.contributor.committeeMember Maxwell, Terry C.
dc.contributor.committeeMember Parkinson, Christopher L.

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